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Fengzhen Zheng,Wei Jiang.An Endoxylanase Rapidly Hydrolyzes Xylan Into Major Product Xylobiose via Transglycosylation of Xylose to Xylotriose or Xylotetraose.DOI: 10.1016/j.carbpol.2020.116121
發布日期:2020-04-28 瀏覽次數:  信息來源:生物學院

An Endoxylanase Rapidly Hydrolyzes Xylan Into Major Product Xylobiose via Transglycosylation of Xylose to Xylotriose or Xylotetraose

Fengzhen Zheng,Lina Song,Abdul Basit,Junquan Liu,Ting Miao,Jiaqi Wen,Yunhe Cao,Wei Jiang


Carbohydr Polymers

DOI: 10.1016/j.carbpol.2020.116121


Abstract

Here, we proposed an effective strategy to enhance a novel endoxylanase (Taxy11) activity and elucidated an efficient catalysis mechanism to produce xylooligosaccharides (XOSs). Codon optimization and recruitment of natural propeptide in Pichia pastoris resulted in achievement of Taxy11 activity to 1405.65 ± 51.24 U/mL. Analysis of action mode reveals that Taxy11 requires at least three xylose (xylotriose) residues for hydrolysis to yield xylobiose. Results of site-directed mutagenesis indicate that residues Glu119,Glu210,and Asp53 of Taxy11 are key catalytic sites, while Asp203 plays an auxiliary role. The novel mechanism whereby Taxy11 catalyzes conversion of xylan or XOSs into major product xylobiose involves transglycosylation of xylose to xylotriose or xylotetraose as substrate, to form xylotetraose or xylopentaose intermediate, respectively. Taxy11 displayed highly hydrolytic activity toward corncob xylan, producing 50.44 % of xylobiose within 0.5 h. This work provides a cost-effective and sustainable way to produce value-added biomolecules XOSs (xylobiose-enriched) from agricultural waste.


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